Table 1 Primer amplification efficiency in RT-qPCR for the candidate reference genes evaluated for normalization of Pleurotus pulmonarius gene expression.
Gene | Description | ID | Primer name | PCR amplification efficiency (%) ± SD |
|---|---|---|---|---|
β-TUB | β-tubulin | 117235 | Pleurotus_pulmonarius_β-TUB | 1.956 ± 0.026 |
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | 1090672 | Pleurotus_pulmonarius_GAPDH | 1.924 ± 0.022 |
VP | Versatile peroxidase | JX021525.1 | Pleurotus_pulmonarius_VP | 1.910 ± 0.024 |
LAC | Laccase | AY836675.1 | Pleurotus_pulmonarius_LAC | 1.893 ± 0.023 |
ACTIN | Actin 1 | 1087906 | Pleurotus_pulmonarius_ACTIN | 1.904 ± 0.026 |
PHOS | Methioadenosine phospharylase | 49987 | Pleurotus_pulmonarius_PHOS | 1.860 ± 0.021 |
EF1α | Elongation Factor 1 | OX344738.1 | Pleurotus_pulmonarius_ EF1α | 1.954 ± 0.023 |
MNP3 | Manganese peroxidase | 1089546 | Pleurotus_pulmonarius_MNP3 | 1.945 ± 0.028 |
Trpho | Trefalose peroxidase | EF645805.1 | Pleurotus_pulmonarius_Trpho | 1.850 ± 0.028 |
MYP | Manganese peroxidase | AB353725 | Pleurotus_pulmonarius_MYP | 1.868 ± 0.024 |
