Table 2 Expression stability of the ten candidate reference genes for normalizing gene expression in Pleurotus pulmonarius cultivated on jatropha cake.

From: Development of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications

Condition

Gene

geNorm

NormFinder

BestKeeper

Rank

M

Rank

SV

Rank

StdDev [CP ±]

Toxic Jatropha cake

β-tubulin

3°

0,139

8°

0,015

_

1.51

Glyceraldehyde 3-phosphate dehydrogenase

7°

0,472

5°

0,014

1°

0.98

Versatile peroxidase

10°

0,642

6°

0,021

_

1.14

Laccase

9°

0,58

4°

0,011

_

1.19

Actin 1

8°

0,522

7°

0,015

_

1.43

Methioadenosine phospharylase

5°

0,317

1°

0,008

_

1.46

Elongation Factor

4°

0,237

3°

0,009

_

1.25

Manganese peroxidase

6°

0,38

2°

0,009

_

1.47

Trefalose peroxidase

2°

0,12

9°

0,015

_

2.11

Manganese peroxidase

1°

0,11

10°

0,016

_

1.46

Non-toxic Jatropha cake

β-tubulin

5°

0,335

5°

0,023

_

1.08

Glyceraldehyde 3-phosphate dehydrogenase

8°

0,581

4°

0,020

1°

0.70

Versatile peroxidase

9°

0,789

2°

0,018

_

1.74

Laccase

1°

0,116

9°

0,032

_

1.34

Actin 1

6°

0,394

10°

0,037

_

1.41

Methioadenosine phospharylase

2°

0,118

3°

0,018

_

1.03

Elongation Factor

3°

0,153

1°

0,015

2°

0.94

Manganese peroxidase

4°

0,267

7°

0,029

_

1.23

Trefalose peroxidase

7°

0,494

6°

0,027

_

1.38

Manganese peroxidase

10°

0,988

8°

0,031

_

1.7

Combined

β-tubulin

4°

0,446

8°

0,028

_

1.25

Glyceraldehyde 3-phosphate dehydrogenase

7°

0,57

4°

0,020

1°

0.85

Versatile peroxidase

9°

0,731

10°

0,040

_

1.69

Laccase

8°

0,617

7°

0,027

_

1.34

Actin 1

5°

0,499

5°

0,023

_

1.43

Methioadenosine phospharylase

2°

0,323

3°

0,018

_

1.30

Elongation Factor

1°

0,31

2°

0,018

_

1.22

Manganese peroxidase

3°

0,358

1°

0,014

_

1.35

Trefalose peroxidase

6°

0,532

6°

0,026

_

1.84

Manganese peroxidase

10°

0,817

9°

0,032

_

1.49

  1. Results were obtained following analyses with the three distinct algorithms geNorm, NormFinder and BestKeeper. GM, geometric mean; M, average expression stability; SV, stability value; Std Dev, standard deviation; Cp, process capability. When employing NormFinder and geNorm, low stability values indicate greater gene expression stability. When employing BestKeeper, genes with standard deviation values greater than 1 are considered as inconsistent.
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