Figure 2 | Scientific Reports

Figure 2

From: Detection of Extended-spectrum β-lactamase-producing Escherichia coli isolates by isothermal amplification and association of their virulence genes and phylogroups with extraintestinal infection

Figure 2

Optimization of the tHDA assay. Agarose gel electrophoresis showed the blaTEM amplicons (A) at 59–67 °C; (B) at 15–90 min; (C) at different DNA template concentrations (0.05–100 ng); and (D) at different primers concentrations (0.025–0.075 µM) [M, 100 bp Marker; C+, positive DNA control; C−, no template control].

Back to article page