Figure 4
Effect of PGE2 on eNOS phosphorylation in experimental NEC pups. (A) Western blot analysis of total eNOS, phosphorylated eNOS, CD31 and VEGFR2 expression in intestinal homogenates of the pups treated as indicated. The representative bands represent blots of a minimum of three independent experiments. Quantitative analysis of total eNOS (B), the phosphorylated eNOS/eNOS ratio (C), CD31 (D) and VEGFR2 (E) are presented as indicated in triplicate. The histograms are represented as the means ± SEMs. *P < 0.01; +#P < 0.01 (one-way ANOVA); (F) Nitric oxide production was assessed in the intestinal segments of the indicated groups. The eNOS inhibitor L-NAME was used to test the eNOS-dependent mechanism during NO measurement. Columns: means; bars, SEM; *P < 0.01; $P < 0.01, and #P < 0.01 (one-way ANOVA).
