Table 1 Summary of the microinsemination experiment.

From: Generation of rat offspring using spermatids produced through in vitro spermatogenesis

No. (%) of oocytes

No. (%) of pups

injected

Survived

Foemed 2PN

Cleaved

Transferred

Implanted

Born

With EGFP

100

76 (76)

67 (88)

6 (8)

76

14 (18)

4 (5)

1 (25)

  1. One hundred oocytes were each injected with a spermatid obtained from the cultured testis tissues. Of these, seventy-six oocytes survived and 67 displayed 2 pronuclei (PN). Cleavage was observed in 6 of these oocytes. The 76 fertilized eggs were then transferred to 3 pseudo-pregnant females. Caesarean section revealed 14 implantation sites and resulted in the birth of 4 live offspring (3 males and 1 female). One of the male pups carried the EGFP transgene.
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