Table 1 Primers for amplification of NS3, and NS5B regions in genotype 3a.

From: Study of drug resistance-associated genetic mutations, and phylo-genetic analysis of HCV in the Province of Sindh, Pakistan

Primer

Sequences

Polarity

Primers for amplification of NS3 region in genotype 3a

 F1

5′-GTCTCTGCRCGATTAGGCCGTGA-3′

Sense strand

 R1

5′-CAGTTTRGCACCAGTTGTAACG-3′,

Antisense Strand

 F2

5′-GTTGGGACCTGCTGATGACTA-3′

Sense strand

 R2

5′-CCCAGTGCGGATGTTGGGGT-3′

Antisense strand

Primers for amplification of NS5B region in genotype 3a

 F1

5′-GTSTGGIARGACYTICTGGAAGAC-3′

Sense strand

 R1

5′-GTSTGGIARGACYTICTGGAAGAC-3′

Anti-sense strand