Table 1 Primers for amplification of NS3, and NS5B regions in genotype 3a.
Primer | Sequences | Polarity |
|---|---|---|
Primers for amplification of NS3 region in genotype 3a | ||
F1 | 5′-GTCTCTGCRCGATTAGGCCGTGA-3′ | Sense strand |
R1 | 5′-CAGTTTRGCACCAGTTGTAACG-3′, | Antisense Strand |
F2 | 5′-GTTGGGACCTGCTGATGACTA-3′ | Sense strand |
R2 | 5′-CCCAGTGCGGATGTTGGGGT-3′ | Antisense strand |
Primers for amplification of NS5B region in genotype 3a | ||
F1 | 5′-GTSTGGIARGACYTICTGGAAGAC-3′ | Sense strand |
R1 | 5′-GTSTGGIARGACYTICTGGAAGAC-3′ | Anti-sense strand |