Figure 1
Development and characterization of rat duodenal organoid. (a) Scheme of the development of rat duodenal organoids. W, Afamin/Wnt3a CM; E, epidermal growth factor; N, Noggin; R, R-spondin 1; A, A83-01; I, IGF-1; F, FGF-2; Y, Y-27632; g, Gastrin, Y0–2 means the supplementation with Y for the first 2 days of culture. (b) The bright-field images of organoids. Scale bar = 50 µm. (c) Whole mount immunostaining of organoids for Sox9 (green) and F-actin (white), Ki-67 (red) and F-actin (white), merge with DAPI (blue), CK20 (green) and P-gp (red) with DAPI (blue), ZO-1 (red) and DAPI (blue), phospho-Ezrin (p-Ezrin, red) and DAPI (blue). Scale bar = 100 µm (d) Day 4 organoids sections were stained with HE and AB-PAS. Scale bar = 100 µm. (e) Transmission electron microscopy of organoids on day 4. Arrows, desmosomes; MG Matrigel, Mv microvilli, Lu Lumen. Scale bar = 2.0 µm.
