Figure 3
Cyst-enriched organoid are suitable for monolayer formation. (a) Scheme of generating the monolayer culture from conventional organoids and cyst-enriched organoids. Day3 or 4 organoids were dissociated into single cells and seeded on Matrigel-coated Transwell (b) The bright-field images of monolayer culture derived from single cells prepared by conventional organoids and cyst-enriched organoids. Cells were stained with basal medium containing 10 µM Y-27632 and 0.5 mg/ml MTT. Scale bar = 1 mm (c) Results of EdU uptake assay of day 1 monolayer culture from single cells prepared by conventional organoids and cyst-enriched organoids. Monolayer cultures were incubated with expansion medium containing 20 µM EdU for 1 h. Scale bar = 100 µm. Data represents mean ± s.d. (n = 4, biological replicates). ***p < 0.001, Student t test. Org Organoid.
