Figure 3 | Scientific Reports

Figure 3

From: Kaempferol-loaded bioactive glass-based scaffold for bone tissue engineering: in vitro and in vivo evaluation

Figure 3

(a) Alizarin red S staining of mineral deposition on ECM in B.SC, C.SC, and KC.SC groups after 21 days incubation (scale bar = 100 μm). (b) ALP activities for B.SC, C.SC, and KC.SC after 7-days and 14-days treatment with conditioned media. Diagrams show the relative expressions of (c) COL1 (Early marker of osteogenic differentiation) and (d) OCN (a late marker of osteogenic differentiation) 14 and 21 days post-incubation in the scaffolds extraction respectively. An asterisk indicates the statistically significant difference between genes and control as measured by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

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