Figure 4
METTL3-dependent upregulation of AREG mRNA by m6A suppressed AREG expression. (A) RIP assay was used to confirm the binding capacity between AREG mRNA and METTL3 in PC cells. (B) The effect of METTL3 knockdown on the m6A levels in AREG in PC cells was analyzed by MeRIP-qPCR. (C) The effect of METTL3 knockdown on the half-life of AREG in PC cells was examined by qPCR. (D) Correlation between AREG mRNA and METTL3 mRNA in PC tissues was analyzed by Spearman. (E) The effects of METTL3 overexpression or knockdown on the expression of AREG mRNA were analyzed by qPCR. (F) The effects of METTL3 overexpression or knockdown on the expression of AREG protein were analyzed by western blotting. Data are expressed as the means ± SD. *P < 0.05, **P < 0.01, and ****P ≤ 0.001 compared with the control. Abbreviations: PC pancreatic cancer, NC negative control, RIP RNA immunoprecipitation, MeRIP Methylated RNA immunoprecipitation, qPCR quantitative polymerase chain reaction, m6A N6-methyladenosine.
