Figure 6
METTL3 is a target gene for miR-3a-3p. (A, B) The target scores of selected miRNAs that bind to the METTL3 gene. miR-33a-3P was selected from the RNAinter and miRDB databases and predicted to have the highest target scores. (C) The expression of miR-33a-3p in 36 pairs of PC tissues collected from our hospital was examined by qPCR. (D) The expression of miR-33a-3p in PC cells and HPNE cells was examined by qPCR. (E) The overexpression of miR-33a-3p was conducted by transfecting PC cells with miR-33a-3p mimics. And the efficacy was analyzed by qPCR. Data are expressed as the means ± SD. *P < 0.05, **P < 0.01, and ***P ≤ 0.001 compared with the control. Abbreviations: PC pancreatic cancer, NC negative control, qPCR quantitative polymerase chain reaction.
