Figure 7
miR-33a-3p downregulates METTL3 expression by directly targeting its 3′-UTR and inhibits proliferation, migration, and invasion. (A, B) The effects of miR-33a-3p mimics on the expression of METTL3 and AREG were detected in PC cells by qPCR (A) and Western blotting (B). (C) The binding sequence of miR-33a-3p on METTL3 mRNA 3′UTR is indicated. (D) Luciferase reporter assay was conducted to examine the binding capacity between miR-33a-3p and METTL3 mRNA 3′UTR in PC cells. (E) The effect of miR-33a-3p mimics on the proliferative capacity in PC cells was examined by CCK8 assay. (F, G) The effects of miR-33a-3p mimics on the migratory (F) and invasive (G) capacity in PC cells were examined by transwell assay. Scale bar: 100 μm. Data are expressed as the means ± SD. ns P > 0.05, *P < 0.05, **P < 0.01, ***P ≤ 0.001, and ****P ≤ 0.001 compared with the control. Abbreviations: PC pancreatic cancer, NC negative control, WT wide type, Mut mutatation, qPCR quantitative polymerase chain reaction, CCK8 cell counting kit-8, 5′-UTR 5′untranslated regions.
