Figure 2 | Scientific Reports

Figure 2

From: Comparing the signaling and transcriptome profiling landscapes of human iPSC-derived and primary rat neonatal cardiomyocytes

Figure 2

Single nuclei PKA activation patterns as observed in hiPSC-CM and RNCM cultures in response to adrenergic agonists and the vasoactive ET-1 peptide. Heatmaps displaying nuclear PKA activity as measured in (A) hiPSC-CMs and (B) RNCMs. Single nuclear PKA data summarized as %ΔF/F (y-axis- each line is a different cell) as a function of time (x-axis). The data was partitioned into four clusters representing distinct nuclear behaviors. Nuclei exhibited either sustained or transient responses to agonists while other nuclei failed to respond or experienced a decrease in activity compared to baseline. Representation of the four response clusters was plotted as a bar chart with percentages of nuclei belonging to each response cluster as observed in (C) hiPSC-CMs and (D) RNCMs. Experiments were performed using RNCMs isolated from 4 different neonatal rat pup litters and 3 independent cardiomyocyte differentiations from hiPSCs. We observed a significant difference between species (p < 0.05) when the hiPSC-CMs or RNCMs were treated with phenylephrine. There is a significant population of cells that experienced a 'negative' response in the hiPSC-CMs (p < 0.05).

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