Figure 3
Single nuclei ERK1/2 activation patterns as observed in hiPSC-CM and RNCM cultures in response to adrenergic agonists and the vasoactive ET-1 peptide. Heatmaps from Supplemental Fig. 7 are plotted in clusters. The three response clusters were plotted as stacked bar charts with percentages of nuclei belonging to each cluster as observed in (A) hiPSC-CMs and (B) RNCMs. Insets reflect sub-clustering applied to the ‘responding’ nuclei cluster. It is apparent that within the ‘responding’ sub-population, two other clusters can be identified based on their responding magnitudes- low or high responders. Experiments were performed using RNCMs isolated from 3 different neonatal rat pup litters and 3 independent cardiomyocyte differentiations from hiPSCs. We observed a significant difference between species (p < 0.05) when the hiPSC-CMs or RNCMs were treated with isoproterenol, in both the responders and non-responder's category. The same was observed when the cells were treated with epinephrine and norepinephrine.
