Figure 4
Single nuclei ERK1/2 activation patterns as observed in hiPSC-CM and RNCM cultures in response to Ang II and β-arrestin biased peptides. The three response clusters were plotted as stacked bar charts with percentages of nuclei belonging to each cluster as observed in (A) hiPSC-CMs and (B) RNCMs. Insets reflect the sub-clustering applied on the ‘responding’ nuclei cluster. It is apparent that within the ‘responding’ sub-population, two other clusters can be identified based on their responding magnitudes- low or high responders. Experiments were performed using RNCMs isolated from 3 different neonatal rat pup litters and 3 independent cardiomyocyte differentiations from hiPSCs.
