Table 2 ΔΔG predictions on protein structure of a subset of identified mutations. Software packages DDGun3D, PremPS, and DynaMut2 utilize input of .pdb structures, though PremPS only accepts X-ray crystallography and not Cryo-EM structures. Software INPS uses sequence information exclusively. ΔΔG values are shaded from blue (+ ΔΔG) to red (− ΔΔG).

From: Attenuated but immunostimulatory Mycobacterium tuberculosis variant bovis strain Ravenel shows variation in T cell epitopes