Figure 2

Peptide profiling of Pru p 3 after gastrointestinal digestion using the high enzyme test performed in the presence of bile salts. Samples were analysed prior to (A,B) and after reduction (C,D). Peptides were mapped onto UniProt accession: Q9LED1. The disulphide bonds are linked by black lines. Linear IgE epitopes are highlighted in pink based on García-Casado (2003). Red arrows: predicted tryptic cleavage sites; blue arrows: predicted chymotryptic cleavage sites. Colour shading represents relative abundance of peptides calculated using spectral counting with the gradient from blue to red indicating increasing abundance.