Table 1 Rate constants and half-life of Pru p 3 and a Mr ~ 7 kDa fragment following gastrointestinal digestion performed under different conditions. ND not determined.
Test type | pH | Bile salts (mM) | Pru p 3 | Mr ~ 7 kDa fragment | |
|---|---|---|---|---|---|
K (min−1) | t½ (min) | K (min−1) | |||
High enzyme | 6.5 | 0 | 0.0022 | 308.6 | ND |
4 | 0.0451 | 15.4 | Transient | ||
8.0 | 0 | 0.0055 | 125.0 | ND | |
4 | 0.1292 | 5.4 | Transient | ||
Low enzyme | 6.5 | 0 | ND | ∞ | ND |
1 | 0.0016 | 427.9 | 0.0126 | ||
8.0 | 0 | ND | ∞ | ND | |
1 | 0.0073 | 94.6 | 0.0216 | ||