Figure 6
ESS2-knockout mice showed reduced VP development. (a) Genotyping PCR of control (Control) and tamoxifen-treated Rosa-CreERT2:Ess2fl/fl (iESS2KO) mice. Primer combinations are described in Supplementary Table. Raw data was shown in Supplementary Fig. 9. (b) Left panel: weights of the VP in Control and iESS2KO mice normalized to body weight. n = 3–4. *, p < 0.05, Student’s t-test. Right panel: representative VPs in Control and iESS2KO mice. Scale bar = 1 mm. (c) HE staining of the VP in Control and iESS2KO mice. Scale bar = 100 μm (20 ×) and 20 μm (40 ×). (d) Representative Ki-67 staining of the VP in Control and iESS2KO mice. The sections were counterstained with hematoxylin. The levels of Ki-67-positive cells decreased in iESSKO mice. Scale bar = 50 μm. (e) Graph of Ki-67-positive cells / total cells from the VP in Control and iESS2KO mice. Five to six independent areas were counted for each of three mice, and the results are presented as means ± standard deviations. *, p < 0.05, Student’s t-test.
