Figure 6 | Scientific Reports

Figure 6

From: Increased body weight in mice with fragile X messenger ribonucleoprotein 1 (Fmr1) gene mutation is associated with hypothalamic dysfunction

Figure 6The alternative text for this image may have been generated using AI.

Decreased activity and numbers of POMC neurons in the arcuate nucleus of the hypothalamus in Fmr1 KO male mice. (a) No difference in the MC4R neuron number in the PVN between WT and KO male mice, quantified as MFI using ImageJ; MFIs of 5–7 coronal sections per mouse were averaged; each dot represents one animal, bar represents group mean ± SEM. (b) KO males have fewer active POMC neurons compared to WT, determined by co-staining of POMC (green, cytoplasmic) and cFOS (magenta, nuclear). cFOS-positive POMC neurons are indicated with arrowheads, inserts contain higher magnification of double positive neurons. Right, numbers of cFOS positive POMC, shown as percentage. (c) KO males have fewer POMC neurons, determined by counting β-endorphin/POMC cell bodies throughout the arcuate nucleus in at least 6 sections per mouse and averaged; each dot represents one animal, bar represents group mean ± SEM. (d) Counts for the rostral and caudal regions demonstrated separately to indicate regional heterogeneity. Statistical significance, indicated with a * (p < 0.05) was determined with t-test followed by Tukey’s post hoc test.

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