Table 2 Positive samples to Flavivirus found in collection performed in 2021.

Year	Week	Method	Collected specimens	Tested specimens	Pool + /N Pool	Virus pool	Species	MLE (95% CI) × 1000	Card + /N Card	Virus Card
2021	30	BG	1278	1238	3/28	USUV	Cx. pipiens	2.71 (0.697–7.256)	0/5	NA
	31	BG	728	708	2/19	USUV	Cx. pipiens (1),	2.89 (0.532–8.757)	0/5	NA
	31	BG	728	708	2/19	USUV	Oc. caspius (1)	2.89 (0.532–8.757)	0/5	NA
	32	BG	1067	1021	4/18	USUV	Cx. pipiens	4.82 (1.502–11.521)	1/5	USUV
	33	BG	1176	1150	4/24	USUV	Cx. pipiens	4.24 (1.314–10.273)	0/5	NA
					3/24	WNV-1	Cx. pipiens	3.01(0.760–8.131	0/5	NA
		CDC	163	159	1/3	USUV	Cx. pipiens	9.87 (0.425–49.063)	NA	NA
	34	BG	900	889	5/25	USUV	Cx. pipiens	6.86 (2.512–14.729)	1/5	USUV
					1/25	WNV-1	Cx. pipiens	1.19 (0.066–5.725)	1/5	USUV
		CDC	254	253	1/4	USUV	Cx. pipiens	5.01 (0.251–23.309)	NA	NA

Collected specimens: total number of collected specimens (all species and both sexes) in week with positive mosquito pools. *Data from BG refers to four consecutive sampling days with five traps, CDC refers to one day of sampling. Tested specimens: total number of tested female target species (Aedes albopictus, Culex modestus, Culex pipiens and Ochlerotatus caspius). N pool + /pool: number of positive pools/number of tested pools. Species: species of mosquitoes in positive pools. MLE (CI): estimated MLE value and 95% Confidence Interval of MLE value. N card + /N card: number of positive FTA cards/number of tested cards.

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