Figure 3
Evaluation of podocyte-related proteins expression on mRNA level within podocytes treated with vitamin D3 or steroid. Different concentrations of vitamin D3 (D3; LOW = 100 nM, HIGH = 1000 nM) and methylprednisolone (MP; LOW = 40 μg/ml, HIGH = 200 μg/ml) were applied to podocytes in 48-h culture. Assessed genes included those coding: nephrin (NPHS1), phosphotyrosine (PTPRO), podocin (NPHS2), and podocalyxin (PODXL). Data are presented as a mean value with standard deviation (mean ± SD) (n = 7). The levels of significant differences were indicated with asterisks or exact p values: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
