Figure 2

Visualization of amplification results from BRMs and red ginseng DNA used to design tetra-primer ARMS-PCR. (A) Amplification results at 28, 35, and 40 PCR cycles. (B) Amplification results with ten-to-one inner vs. outer primer ratio at 40 cycles. (C) Amplification results with ten-to-one inner versus outer primer ratio and deliberate mismatch at − 2 position at 40 cycles. Green arrow: faint PCR product at 28 cycles. Red circles: decreased band intensity at 35 and 40 cycles versus 28 cycles. Blue arrows: non-specific amplification of diagnostic bands. Red arrows: non-specific amplification of unexpected bands.