Table 2 Triplicate absorbance readings (and average reading) and the calculated concentration of proteins in the analyte (diluted for measurement) and the original depleted serum sample.
Sample | Absorbance 1 | Absorbance 2 | Absorbance 3 | Average absorbance | Analyte concentration (μg/μL) | Sample concentration (μg/μL) |
|---|---|---|---|---|---|---|
A1 | 0.428 | 0.431 | 0.444 | 0.434 | 0.424 | 4.244 |
A2 | 0.375 | 0.378 | 0.395 | 0.383 | 0.374 | 3.740 |
A3 | 0.349 | 0.353 | 0.36 | 0.354 | 0.346 | 3.461 |
A4 | 0.417 | 0.415 | 0.432 | 0.421 | 0.412 | 4.117 |
B1 | 0.3 | 0.307 | 0.315 | 0.307 | 0.301 | 3.005 |
B2 | 0.321 | 0.326 | 0.339 | 0.329 | 0.321 | 3.213 |
B3 | 0.319 | 0.317 | 0.333 | 0.323 | 0.316 | 3.158 |
C1 | 0.321 | 0.316 | 0.326 | 0.321 | 0.314 | 3.139 |
C2 | 0.317 | 0.316 | 0.328 | 0.320 | 0.313 | 3.132 |
C3 | 0.335 | 0.328 | 0.337 | 0.333 | 0.326 | 3.259 |