Figure 4

Dot blot for Isolated exosomes by molecular exclusion chromatography was performed on 5 plasma samples of patients with gastric cancer. Each fraction obtained was probed for Tetraspanins CD9, CD63, TSG-101, and for HLA-G. The expression of these proteins can be observed from Fraction 6 to 12, indicating the presence of exosomes as reported for this technique. dot blot.