Figure 4
Immunostaining for GFAP and MAP2 proteins in FCD IIa and IIb tissues. Representative images of cortical immunostaining, DAB channel conversion, and grayscale conversion for (A–F) the astrocyte marker GFAP (glial fibrillary acidic protein), and (H–K) the neuron marker MAP2 (microtubule-associated protein 2). Barplots of GFAP (G) and MAP2 (N) expression levels in FCD IIa (n = 4) and IIb (n = 4) lesions. The mean expression was averaged from 10 randomly selected subfields in each lesion. The uncertainty bars represent 95% confidence intervals of the means. The image grayscale conversion of the positive immunostained area was used for marker quantification. Wilcoxon rank-sum test, ****p < 10−4. Scale bar: 50 μm.
