Figure 5
Restoration of growth when escin is pre-mixed with ergosterol, but not brassicasterol. (a) Structure of ergosterol (the predominant sterol in S. cerevisiae plasma membrane), brassicasterol (a plant sterol), and sterols which have been identified as accumulating in the erg3Δ strain (episterol, ergosta-7,22-dienol, ergosta-7-enol). (b) and (c), Growth in microplate cultures indicated by ΔA595 (0–24 h). Three biological replicates ± standard deviation (SD). In (b), the MIC of escin in CSM media is 63 μg/mL for WT, which is 2.4-fold lower than in rich complex medium. In (c), WT cells were grown in the presence of 0 or 63 μg/mL escin (1.25% methanol), alone or pre-mixed with ergosterol or brassicasterol in a 1:1 molar ratio. Ergosterol and brassicasterol only controls were included. Statistics: one-way ANOVA with post-hoc Tukey HSD test, conditions not connected by the same letter are significantly different (p ≤ 0.05).
