Figure 1

Sortilin and syndecan-1 immunolabelling depicts pathogenesis of PCa, which aligns with immunofluorescence in prostate cell line models. (a) Representative serial tissue sections stained with hematoxylin and eosin (H&E), showing benign glands, Gleason pattern 3 and Gleason pattern 4. (b) Benign glands, Gleason pattern 3 and Gleason pattern 4 were IHC-labelled for sortilin and (c) for syndecan-1. (d) Representative maxIP confocal images, showing immunolabelling of prostate cell lines with anti-sortilin antibodies and (e) anti-syndecan-1 antibodies. Scale bars; 10 µm. (f) Quantification of sortilin and syndecan-1 fluorescence intensity in prostate cell lines. Data are presented as mean ± SD, n = 3 (independent experiments), one-way ANOVA test. Scale bar for tissue images (a–c)is depicted in (c); 100 µm.