Figure 2
From: Dynamic interplay between sortilin and syndecan-1 contributes to prostate cancer progression
Sortilin controls glucose uptake in PCa cells and glucose alters expression of sugar metabolism-related proteins. (a) Sortilin (SORT1) and syndecan-1 (SDC1)-interacting proteins, which are implicated in PCa development and progression. Red nodes; SORT and SDC1, gray nodes; proteins crucial for PCa metabolism relevant to this study. Line thickness indicates the strength of data support. (b) diagram showing involvement of sortilin (SORT) in GLUT4 and potentially GLUT1 pathways. (c) Expression of GLUT1, (d) GLUT4 and (e) glucose uptake in LNCaP cells after sortilin knockdown (siSORT) as compared to scrambled siRNA (Scr siRNA). (f) Expression of SORT, GLUT1 and GLUT4 in different glucose concentrations in prostate cell lines. Western blotting signal was normalised using total protein staining. Data are presented as mean ± SD, in (c,d,f) n = 3 (independent experiments), in (e) n = 5 (independent experiments), in (c–e), one-sample t-test, in (f) one-way ANOVA test, where samples were normalized and compared to 5.5 mM glucose.
