Figure 6

Androgens regulate sortilin (SORT)-mediated glucose metabolism in LNCaP cells. (a) Glucose uptake by LNCaP cells treated with vehicle (Veh) versus R1881. (b) Representative maxIP confocal images of LNCaP cells immunolabelled with anti-SORT antibodies. Scale bars; 10 µm. (c) Expression of SORT and a corresponding quantification of band densities. Western blotting signal was normalised using total protein staining. (d) Quantification of GLUT1 fluorescence intensity. (e) Representative confocal images showing distribution of GLUT1. Scale bars; 20 µm. (f) Representative confocal images of cells co-labelled with anti-GLUT1 (red) and anti-SORT (green) antibodies. Scale bars; 10 µm. (g) Quantification of colocalisation between GLUT1 and SORT. (h) Quantification of Rab4A fluorescence intensity. (i) Quantification of colocalisation between Rab4A and GLUT1. (j) Quantification of Rab11A fluorescence intensity. (k) Quantification of colocalisation between Rab11A and GLUT1. (l) Quantification of GLUT4 fluorescence intensity. (m) Representative confocal images of cells co-labelled with anti-GLUT4 (red) and anti-SORT (green) antibodies. Scale bars; 10 µm. (n) Quantification of colocalisation between GLUT4 and SORT. (o) Expression of AS160 and a corresponding quantification of band densities. Western blotting signal was normalised using total protein staining. Data are presented as mean ± SD, in (a,c,d,h,j,l,o) n = 3 (independent experiments), in (g,i,k,n) n = 10 representative ROIs, in a one sample t-test, all other graphs; t-test.