Figure 1 | Scientific Reports

Figure 1

From: Different levels of autophagy induced by transient serum starvation regulate metabolism and differentiation of porcine skeletal muscle satellite cells

Figure 1The alternative text for this image may have been generated using AI.

Detection of autophagy-related protein expression by WB. (A) WB results of LC3B-II and P62; (B) Grayscale analysis of LC3B-II/LC3B-I; (C) Grayscale analysis of P62; (D) WB results of autophagy signal pathway proteins AMPK, p-AMPK, mTOR and p-mTOR; (E) Grayscale analysis of P-mTOR/mTOR; (F) Grayscale analysis of p-AMPK/AMPK. The gel images of mTOR and AMPK were cropped from the same membrane. The blots were cut prior to hybridisation with antibodies. The data are expressed as the mean ± SD (n = 3) of three experiments. The statistical significance of differences between groups was analyzed using two-tailed Student’s t-tests, and differences were considered statistically significant when *P < 0.05 and **P < 0.01 (Supplementary information 1).

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