Figure 4

Hepatocyte apoptosis was relieved by intermittent fasting. (A) Following the 22-week intervention, TUNEL staining was performed in liver slices, scale bar = 100 μm; (B) The TUNEL positive cell numbers were analyzed; (C,D) The protein expression of BAD, BAX, and Bcl-2 in the liver were determined and analyzed; Data are expressed as mean ± SD, n = 3–6. *, ** represent P < 0.05, P < 0.01.