Figure 4

Expression of tomato WRKY genes in response to M. javanica infection. Expression of SlWRKY16 (a) and SlWRKY31 (b) genes in tomato roots was determined by qRT-PCR at 1, 2, 3, 10 and 15 dpi and in uninfected control root segments (0 dpi). Data include three independent biological and three technical replicates. WRKY16 and WRKY31 expression values are relative to uninfected control roots and were normalized using tomato β-tubulin as a reference gene. Bars indicate SEM. Different letters above the bars indicate statistically significant differences based on Tukey's HSD test (P < 0.05).