Figure 6 | Scientific Reports

Figure 6

From: SlWRKY16 and SlWRKY31 of tomato, negative regulators of plant defense, involved in susceptibility activation following root-knot nematode Meloidogyne javanica infection

Figure 6

SlWRKY16 overexpression in tomato hairy roots enhances M. javanica nematode infestation. Increased susceptibility of tomato hairy roots overexpressing SlWRKY16 (WRKY16-OE-E2 and WRKY16-OE-E5) expressed as increased infection reflected by number of (a) eggs and (b) galls compared to the control line (empty vector). (c) Susceptibility of tomato roots overexpressing SlWRKY16 to nematodes compared to control line. All root lines were subjected to inoculation with 500 sterile preparasitic J2s, and the infected roots were examined 28 days post-infection (dpi) to evaluate gall and egg development using a dissecting microscope. It is worth noting the significant (P < 0.05) increase in the percentage of eggs and galls in the WRKY16-OE-E2 and WRKY16-OE-E5 root lines compared to the control group. The data are presented as the means of 15 plants from each line, and the experiment was repeated three times, yielding consistent outcomes. The SEM represents the percentage of each developmental stage. Different letters above the bars indicate significant differences (P < 0.05, ANOVA) among the hairy root lines, as determined by Tukey–Kramer multiple comparison tests.

Back to article page