Figure 4

SynTOF analysis reveals a strong proximity between Hu and NHP presynaptic events with species specificity at the protein level. (A) t-SNE of presynaptic events after nonlinear dimension reduction colored by clusters. (B) Visualization of the connection between mean expression of Hu and NHP samples from different clusters. Nodes represent mean expression vectors per cluster stratified by species, edges correspond to Pearson correlation between nodes vectors. Only significant edges (P-value < 0.05 using non-correlation testing) above the global mean edge value are displayed. Homogeneity was observed across the two datasets, suggesting a high similarity between identified presynaptic events in the two species. Node positions were computed based on the Fruchterman-Reingold algorithm. (C) Original single events from neostriatum embedded and projected in two dimensions using t-SNE, colored by species. (D) Mean frequency of Hu and NHP synaptic events per cluster after removing events present in less than 0.01. Symbols indicate significant differences using Wilcoxon’s P-value < 0.05 (*) after Benjamini–Hochberg correction. (E) Volcano plot of differential protein expression between Hu and NHP, per cluster in cerebral cortex and neostriatum after multiple testing corrected Wilcoxon’s test. Only significantly different marker expressions are colored.