Figure 3
Analytical sensitivity of TP LAMP assay. (A) Amplification curves for ten-fold serially diluted DNA of T. pecoris DSM 111392T. Characteristic for LAMP, the amplification curves flattened with decreasing DNA concentration. NTC = non-template control. (B) Detection times for ten-fold serially diluted DNA of T. pecoris DSM 111392T as a function of DNA concentration per reaction. Marks and error indicator bars represent the mean and standard deviation, respectively, of three independent measurements. With decreasing DNA concentration, detection times did not increase linearly. For this reason, LAMP was not suitable for quantifying the target species.
