Figure 5

In vivo ICG fluorescence of BSA, DW, dextrose injection, and saline with the same ICG concentration (20 μg/mL) in the lymphatic system. (A) Incision area for observation of brachial lymph nodes (LNs) and collecting lymphatic vessels (LVs) connected to them. (B) ICG fluorescence images after injection of each ICG solution (inset). (C) The normalized ICG intensity of the cross-sectional profile of LNs (yellow dot lines in B) and LVs (red dot lines in B). The gray area graphs were derived from the visible images of the LNs and LVs verified using Evans blue injection (Suppl. Fig. 2B). The outcome values were derived by taking the average of three measurements (n = 4).