Figure 2
From: Mechanical stimuli activate gene expression via a cell envelope stress sensing pathway
VxrAB signaling responds to mechanical stress from compression and hydrostatic pressure. (A) During compression, cells are sandwiched between agarose gel and a weighted glass slide. (B) Single cell fluorescence of ΔcrvA PmurJ:msfGFP cells (pink, n = 1130, 1160, 1304 in order of loading), ΔcrvA ΔvxrB box PmurJ:msfGFP cells (blue, n = 1461, 1189, 1304), and (C) ΔcrvA PvxrAB:msfGFP cells (green, n = 1541, 1058, 1826) vs applied compression force. Solid lines are linear regressions. The slope of ΔcrvA PmurJ:msfGFP cells (pink) is greater than the slope of the ΔcrvA ΔvxrB box PmurJ:msfGFP cells (blue) (p < 0.001). (D) Hydrostatic pressure applies force equally to all surfaces of the cell compressing the cell envelope. (E) Single cell fluorescence of ΔcrvA PmurJ:msfGFP cells (pink, n = 3845, 2261, 1393), ΔcrvA ΔvxrB box PmurJ:msfGFP cells (blue, n = 2085, 1194, 1554). The slope of ΔcrvA PmurJ:msfGFP cells (pink) is greater than the slope of the ΔcrvA ΔvxrB box PmurJ:msfGFP cells (blue) (p < 0.001). (F) ΔcrvA PvxrAB:msfGFP cells (green, n = 2332, 1971, 907) vs hydrostatic pressure. Solid lines are linear regressions.
