Figure 6 | Scientific Reports

Figure 6

From: Cardamonin protects against iron overload induced arthritis by attenuating ROS production and NLRP3 inflammasome activation via the SIRT1/p38MAPK signaling pathway

Figure 6

SIRT1 inhibitor EX527 reverses the inhibitory effect of CAR on FAC-induced chondrocyte apoptosis and NLRP3 inflammasome secretion. (a,b) Chondrocytes were treated with 100 μm FAC and co-cultured with 10 μm EX527, 5 μm CAR, 5 μm CAR + 10 μm EX527. And p38, p-p38, SIRT1, IL-18, IL-1β, NLRP3 protein levels were observed using protein blotting and the statistical results are shown in the bar graphs. (c,d) In addition, we examined changes in chondrocyte apoptosis under the same culture conditions using Annexin V-FITC/PI dual-labeling flow cytometry (data are shown as standard deviation ± mean; # P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05 vs. Mod).

Back to article page