Figure 1

Synthesis of SeNPs and preparation of selenium-enriched B. amyloliquefaciens C-1. The 1, 3, 5, 7, 10% fresh overnight culture of C-1 were sub-cultured in LB + G medium with 0, 15, 30, 60, 100, 150 μg/ml selenite, separately. And the growth of C-1 was monitored by measuring OD600 every 60 min at 30 °C for 22 h (A); the fermentation culture of C-1 in LB + G medium with 100 μg/ml Na2SeO3 at 0, 6, 12, 18, 24 and 30 h (B); selenium conversion rate of C-1 in LB + G medium with 100 μg/ml Na2SeO3 and with 10% inoculum, the cells were collected every 6 h. Selenium were determined by spectrophotometric method (C).