Figure 2 | Scientific Reports

Figure 2

From: PDI augments kainic acid-induced seizure activity and neuronal death by inhibiting PP2A-GluA2-PICK1-mediated AMPA receptor internalization in the mouse hippocampus

Figure 2

Effects of PDI knockdown on GluA2 phosphorylation and surface AMPAR expression in the hippocampus under physiological condition. As compared to control siRNA, PDI siRNA enhances GluA2 S880, but not Y869/873/876, phosphorylation in the mouse hippocampus. GluA2 S880 upregulations are observed in the dendrites and cell bodies of hippocampal neurons. However, PDI siRNA reduces surface expression of GluA1 and GluA2 without affecting surface GluA1/GluA2 ratio. (a) Representative Western blot images for GluA2, p-GluA2 Y869/873/876 and p-GluA2 S880 levels. (b–d) Quantitative analyses of the effects of PDI siRNA on GluA2 (b), p-GluA2 Y869/873/876 (c) and p-GluA2 S880 levels (d) based on the Western blot data (*p < 0.05 vs. control siRNA; n = 7, respectively; Mann–Whitney test). (e) Representative double immunostaining for NeuN and GluA2 S880 in CA1 and CA3 pyramidal neurons. (f) Representative Western blot images for surface expressions of GluA1 and GluA2. (g–i) Quantitative analyses of the effects of PDI siRNA on surface GluA1 (g) and GluA2 (h) levels and surface GluA1/GluA2 ratio (i) based on the Western blot data (*p < 0.05 vs. control siRNA; n = 7, respectively; Mann–Whitney test).

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