Figure 4 | Scientific Reports

Figure 4

From: PDI augments kainic acid-induced seizure activity and neuronal death by inhibiting PP2A-GluA2-PICK1-mediated AMPA receptor internalization in the mouse hippocampus

Figure 4

Effects of PDI knockdown on PDI bindings to PP1 and PP2A, and the thiolization and S-nitrosylation and on PP2A in the hippocampus under physiological condition. As compared to control siRNA, PDI siRNA reduces PDI:PP2A bindings without affecting PDI:PP1 bindings. In addition, PDI knockdown reduced the amount of total thiols on PP2A, while it does not affect that of SNO-thiols in the mouse hippocampus. (a) Representative Western blot images for the PDI:PP1 bindings. (b–c) Quantitative analyses of the effects of PDI siRNA on PP1 level (b) and PDI:PP1 binding (c) based on the Western blot data (n = 7, respectively). (d) Representative Western blot images for the PDI:PP2A bindings. (e–f) Quantitative analyses of the effects of PDI siRNA on PP2A level (e) and PDI:PP2A binding (f) based on the Western blot data (*p < 0.05 vs. control siRNA; n = 7, respectively; Mann–Whitney test). (g) Representative Western blot images for the thiolization and S-nitrosylation on PP2A. (h–i) Quantitative analyses of the effects of PDI siRNA on thiolization (h) and S-nitrosylation on PP2A (i) based on the Western blot data (*p < 0.05 vs. control siRNA; n = 7, respectively; Mann–Whitney test).

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