Figure 6

TRPV2 affects the osteoclast differentiation, but not the proliferation, of RAW264.7 cells. RAW264.7 cells were cultured in RANKL and treated with vehicle (DMSO) or 0 mM (a), 50 mM (b), 75 mM (c), or 100 mM (d) tranilast for 3 days (a–d). The cells were stained for TRAP activity. Scale bar, 200 µm. Quantification of TRAP-positive multinucleated cells generated from RAW264.7 cells treated with vehicle or tranilast for 3 days (e). ^ap < 0.01 vs. 0 mM; ^bp < 0.01 vs. 50 mM; ^cp < 0.05 vs. 75 mM (n = 4). Proliferation of RAW264.7 cells evaluated using Cell Count Reagent SF (f). There was no significant difference in cell number between the vehicle and tranilast groups (n = 4). RAW264.7 cells were cultured with RANKL and treated with vehicle or 50 mM tranilast for 3 (g–i,m,o) or 5 days (j–l,n,p). mRNA levels of NFATc1 (g,j), cathepsin K (h,k), TRAP (i,l), TRPV4 (m,n), and TRPV2 (o,p), as determined by qPCR. ^ap < 0.01 vs. vehicle (n = 4).