Figure 2
From: Discrepancies in indel software resolution with somatic CRISPR/Cas9 tumorigenesis models
Mutational landscape correlates with the variability of indel analysis software. (A) Total indel percentage of Nf1 sequences on Synthego, TIDE, and DECODR (n = 18). Variability in total indel percentage was analyzed by one-way ANOVA with Tukey’s multiple comparisons. (B,D) Global indel characterization of number of indels (B), indel type (C), and maximum indel size detected (D). (E) Representative indel characterization of a Nf1 sequence with a simple mutational landscape of 1–2 indels detected. (F) Representative indel characterization of a Nf1 sequence with a complex mutational landscape of ≥ 3 indels detected. (G) Global number of indel characterization of sequences that had greater than 20% variability across platforms. (H) Global indel size characterization of sequences that had greater than 20% variability across platforms. Data represents biological replicates with the mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
