Figure 3

Analysis of PET imaging. (a) CT and PET/CT images with [⁶⁸Ga]Ga-THP-Pam (in rows indicated by red bars) and [¹⁸F]NaF (in rows indicated by blue bars) 60–120 min post-injection. The panels show (from top to bottom): axial view of the heart and lungs; axial view of the stomach and kidneys; coronal view of the abdomen; sagittal view of the abdominal spine and aorta. CT and PET scales are matched for each view. (b) Quantified PET data of the %IA in each organ of interest ([⁶⁸Ga]Ga-THP-Pam: n = 4; [¹⁸F]NaF: n = 3). The entire organ has been included in the ROI, and stomach data from rats fed a healthy diet has been excluded from the data as accurate ROIs could not be drawn due to the stomach not being visible in the images. Data are tabulated in Table S1 (Supplementary Information). (c) Ex vivo light images (top) and pseudo-coloured autoradiography (bottom) of abdominal sections from rats injected equal volumes of the same batch of [⁶⁸Ga]Ga-THP-Pam and culled 2 h post-injection, showing higher uptake in the aorta of rats fed the EC diet than in rats fed the healthy diet. A aorta, B bladder, H heart, K kidneys, LI large intestine, Ln lungs, Lv liver, S stomach, SI small intestine.