Table 1 Kinetic parameters of the NADH and NADPH peroxidases from intestinal sulfate-reducing bacteria*
From: NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria
Kinetic parameters | D. piger Vib-7 | D. orale Rod-9 | ||
|---|---|---|---|---|
NADH | NADPH | NADH | NADPH | |
Exponential growth phase | ||||
V0 (µmol × min-1 × mg−1 protein) | 141 ± 13 | 289 ± 27 | 127 ± 11 | 144 ± 13 |
Pmax (µmol × mg−1 protein) | 51 ± 4 | 43 ± 3 | 38 ± 3 | 27 ± 2 |
τ (min) | 0.36 ± 0.03 | 0.15 ± 0.012 | 0.30 ± 0.017 | 0.19 ± 0.017 |
Vmax (µmol × min−1 × mg−1 protein) | 66 ± 5 | 61 ± 5 | 58 ± 6 | 70 ± 6 |
Km (mM) | 0.97 ± 0.08 | 1.21 ± 0.11 | 1.58 ± 0.13 | 4.76 ± 0.44 |
Stationary growth phase | ||||
V0 (µmol × min−1 × mg−1 protein) | 828 ± 7 | 343 ± 24 | 368 ± 34 | 103 ± 9 |
Pmax (µmol × mg−1 protein) | 84 ± 7 | 43 ± 4 | 51 ± 4 | 32 ± 2 |
τ (min) | 0.10 ± 0.09 | 0.13 ± 0.01 | 0.14 ± 0.01 | 0.31 ± 0.02 |
Vmax (µmol × min−1 × mg−1 protein) | 132 ± 12 | 69 ± 5 | 78 ± 6 | 70 ± 6 |
Km (mM) | 1.42 ± 0.11 | 1.49 ± 0.13 | 1.02 ± 0.10 | 3.24 ± 0.31 |
