Figure 8
(a) DNA protective potential of SP5P compound purified from SP5 against reactive oxygen species generated by Fenton’s reagent. Well 1 (negative control): only plasmid DNA; Well 2: Fenton’s reagent; Well 3: Positive control (rutin, 10 μg); Wells 4–7: Fenton’s reagent + different concentrations of SP5P compound (5, 10, 15 and 20 μg/well); Supercoiled (Form I); Single strand nicked DNA (Form II); Linear (Form III); (b) Densitometric analysis of pBR322 plasmid DNA treated with SP5P compound in the presence of Fenton’s reagent (original gel are presented in Supplementary Fig. X6).
