Figure 3
From: Synthetic circuits based on split Cas9 to detect cellular events
Logic gate for sensing cells that are undergoing EMT. Representative images of H1299 lung cancer cells (A) and H2170 VRCs cells (B), both partly mesenchymal. Both cell lines, when exposed to TGFβ (+ TGFβ) as an EMT enhancer, increased the number of EGFP expressing cells as a proof of undergoing EMT. Morphological changes were visible after activation of TGFβ showed features of cells of mesenchymal origin—more spindle-shaped, they had more protrusions facilitating migration in comparison to controls.
