Figure 4
From: Synthetic circuits based on split Cas9 to detect cellular events
A two level AND logic gate, where epithelial cancer cells undergoing EMT are detected. Four epithelial cancer cell lines were transfected with split Cas9/gRNA system and the EGxxFP reporter. Then cells were stimulated to undergo EMT by the addition of TGFβ. While all these epithelial cancer cells were known to express the phCEA (Fig. 2), only cells with partial-EMT expressed the pTBD-Cas4,5C, without EMT stimulation (upper row, -TGFβ). But when EMT was induced (+ TGFβ), the number of positive cells increased dramatically in those partially mesenchymal (H1299 and H2170), and appeared in those more epithelial—HeLa and U-2 OS cells (lower row).
