Figure 2
From: Caffeine exacerbates seizure-induced death via postictal hypoxia
Drug targets of adenosine A1 and A2A receptor subtype. (A) Model schematic: following the injection of vehicle or drug, cardiorespiratory (HR: heart rate/ RR respiratory rate), oxygen and LFP signals were measured. electromyograph (EMG) electrode wires record respiratory activity directly from diaphragm and electrodes record cardiac activity. A bipolar electrode in the brainstem records local field potential (LFP) in the pre-Bötzinger complex (PBC) and an oxygen sensing optode in the contralateral PBC records absolute oxygen. Mean time to death in male C57BL6/J mice treated with drugs targeting adenosine receptor subtypes. (B) Vehicle mice (n = 11) mean time to death was 50.44 ± 3.95, Caffeine mice (n = 11) mean time to death was 27.21 ± 2.65 (t20 = 4.87, p =  < 0.0001****). (C) Vehicle mice (n = 10) mean time to death was 51.80 ± 3.17, Theophylline (n = 10) mean time to death was 30.32 ± 3.67 (t18 = 4.42, p = 0.0003***). (D) Vehicle mice (n = 10) mean time to death was 49.12 ± 2.44, N6 (n = 10) mean time to death was 35.95 ± 4.27 (t18 = 2.67, p = 0.0154*). (E) Vehicle mice (n = 10) mean time to death was 19.12 ± 2.44, CGS-21680 (n = 10) 114.0 ± 5.72 (t18 = 10.42, p =  < 0.0001****). Histobars represent mean ± SEM.
